Refine your search
Collections
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Agastian, Paul
- Lignin Degradation:A Microbial Approach
Abstract Views :231 |
PDF Views:26
Authors
Affiliations
1 Research Department of Plant Biology and Biotechnology, Loyola College, Chennai-34, Tamil Nadu, IN
2 Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, SA
1 Research Department of Plant Biology and Biotechnology, Loyola College, Chennai-34, Tamil Nadu, IN
2 Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, SA
Source
South Indian Journal of Biological Sciences, Vol 1, No 3 (2015), Pagination: 119-127Abstract
Lignin is the most structurally complex carbohydrate consisting of various bilogically stable linkages. It has been estimated that lignin constitutes 30-35% of the earth's non-fossil organic carbon. Lignin degradation plays an important role in carbon recycling and biofuel production. Industrial waste treatment through chemical approaches is not only expensive but also toxic to environment. Lignin degrading enzymes from microbes contribute a major role in the degradation of industrial effluents. Fungi and bacteria are good sources of effluent hydrolyzing enzymes such as lignin peroxidase, laccase, manganese peroxidase etc. However, there are very few research activities regarding role of microbes in lignin degradation. Lignin hydrolysis using various microbes with eco-friendly approach is a challenge for researchers globally. Therefore, in order to reduce the adverse effect of industrial effluent on environment, the quest for inexpensive, sustainable and eco-friendly approach is indispensable. The current review focuses to enlighten a comprehensive and broad analysis of lignin degradation ability of various fungal and bacterial sources using their ligninolytic machinery system.Keywords
Bacteria, Biodegradation, Fungi, Ligninolytic Enzymes, Lignocellulose.Full Text
- In vitro Regeneration of a Rare Antidiabetic Plant Epaltes divaricata L.
Abstract Views :296 |
PDF Views:12
Authors
Affiliations
1 Research Department of Plant Biology and Biotechnology, Loyola College, Chennai - 600 034, IN
1 Research Department of Plant Biology and Biotechnology, Loyola College, Chennai - 600 034, IN
Source
South Indian Journal of Biological Sciences, Vol 1, No 1 (2015), Pagination: 52-59Abstract
This study was conducted to develop an efficient protocol for in vitro direct and indirect regeneration of Epaltes divaricata L. MS (Murashige and Skoog) medium was supplemented with different growth hormones both individually and in combinations. MS medium containing BAP (1.0 mg/L) and TDZ (0.1 mg/L) in combination proved to be most effective in inducing maximum callus with multiple shoot formation using shoot tip from the explants producing maximum of 10.77±0.4 shootlets / explants with mean length of 4.30±0.3 cm from leaf explants. Regenerated multiple shoot induction using nodal explants supplemented BAP (1.0 mg/l) with NAA (0.1 mg/L) produced 1.86±1.05 average number of shoots / explants, with mean length of shoots 3.00±0.64 cm whereas BAP (1.0 mg/L) with KN (0.1 mg/L) produced 4.16±0.36 average number of shoots with mean length of shoot 3.08±0.33 cm. The leaf explants produced good callus in the combination of growth regulator BAP (0.5 mg/L) and NAA (0.1 mg/L) 80% response and number of shoot per callus is 3.38 ± 0.21. In vitro ischolar_maining of micro propagated shoot response in MS media with IBA (0.1 mg/L) producing more ischolar_mains (13.31±0.24 numbers) with ischolar_main length 7.16±0.51 cm followed by NAA (0.1 mg/L) producing 8.21±0.26 numbers with ischolar_main length 5.22±0.41 cm. Regenerated of Epaltes divaricata L. plantlets were hardened in organic manure with sand showed acclimatization percentage is 73.33 % after 2 months in the field.Keywords
MS Medium, Multiple Shoots, Regeneration, Hardening, Epaltes divaricata L.Full Text
- Isolation and Molecular Characterization of Xylitol Producing Wild Yeast Strains from Different Fermented Fruit Juices
Abstract Views :237 |
PDF Views:14
Authors
Affiliations
1 Research Department of Plant Biology and Biotechnology, Loyola College, Nungambakkam, Chennai-34, Tamil Nadu, IN
1 Research Department of Plant Biology and Biotechnology, Loyola College, Nungambakkam, Chennai-34, Tamil Nadu, IN
Source
South Indian Journal of Biological Sciences, Vol 2, No 4 (2016), Pagination: 415-423Abstract
This study was aimed to isolate potential yeast species from commercial fruits available in different places of Chennai, India. Fruit juice samples were cultured in yeast extract dextrose (YED) media. A total of 33 yeast isolates originating from fruit juice were identified by molecular approaches and screened for the potential yeast for fermenting condition for xylitol production. For detailed and final identification, the ITS-PCR products were subjected to sequencing followed by Blast analysis. These genera including Candida (13 isolates), Pichia (7 isolates), Meyerozyma (5 isolates), Saccharomycetes (2 isolates), Hanseniaspora (3 isolates), Saccharomycetales (2 isolates) and Debaryomyces (1 isolates) as well as three related anamorphic species assigned to phylogenetic analyzed frominternal transcribed spacer (ITS) region. Based on biochemical test and xylanase assay, out of 33 isolates, six isolates showed maximum enzyme activity. Among 6 isolates, Candida tropicalis strain LY15 showed above >300 colony xylose utilization test. HPLC analysis confirmed the xylitol production from Candida tropicalis strain LY15, indicating the potential role of the isolate at industrial scale.Keywords
Fruits Juice, Yeast Strains, Xylose Assimilation, Phylogenetic Analysis.Full Text
References
- Vandeska E, Amartey S, Kuzmanova S, Jeffries TW. (1995). Effects of environmental conditions on production of xylitol by Candida boidinii. World Journal Microbial Biotechnology, 11, 213–218.
- Zagustina NA, Rodionova NA, Mestechkina NM, Shcherbukhin VD, Bezborodov AM. (2001). Formation of xylitol in Candida guilliermondii 2581 culture. PriklBiokhimMikrobiology, 37, 573–577.
- Rodrigues RC, Felipe MG, Roberto IC., Vitolo M. (2003). Batch xylitol production by Candida guilliermondii FTI 20037 from sugarcane bagasse hemicellulosic hydrolyzate at controlled pH values. Bioprocess BiosystemEngineering, 26, 103–107.
- Oh DK, Kim SY, Kim JH. (1998). Increase of xylitol production rate by controlling redox potential in Candida parapsilosis. Biotechnology Bioengineering, 58, 440–444.
- Saha B and Bothast R. (1999). Production of xylitol by Candida peltata. Journal Indian Microbial Biotechnology, 22, 633–636.
- Kim JH, Han KC, Koh YH, Ryu YW, Seo JH. (2002) Optimization of fed-batch fermentation for xylitol production by Candida tropicalis. Journalof Indian Microbial Biotechnology, 29, 16–19.
- Lopez F, Delgado OD, Martinez MA, Spencer JF, Figueroa LI. (2004). Characterization of a new xylitol-producer Candida tropicalis strain. Antonie Van Leeuwenhoek, 85, 281–286.
- Saigal D. (1994). Isolation and selection of thermotolerant yeasts for ethanol production. Indian Journalof Microbiology. 34(3), 193-203.
- Aro N, Pakula T, Penttilä M. (2005). Transcriptional regulation of plant cell wall degradation by filamentous fungi. FEMS Microbiology Review. 29(4), 719-739.
- Margolles-Clark E, Ihnen M, Penttilä M. (1997). Expression patterns of ten hemicellulase genes of the filamentous fungus Trichodermareesei on various carbon sources. Journal of Biotechnology. 57(1), 167-179.
- Kurtzman CP, Fell JW, Rosa CA, Peter G (2006). Yeast systematics and phylogeny - implications of molecular identification methods for studies in ecology. The Yeast Handbook. Germany: Springer-Verlag, Berlin-Herdelberg, pp. 11-30.
- Barnett JA, Payne RW, Yarrow D (1990). “Yeasts: Characteristics and identification”. Cambridge University Press, Cambridge, 2nd. Ed., p.1002.
- Kreger-van Rij NJW. 2013. The yeasts: a taxonomic study. Elsevier.
- Jeyaram K, Singh WM, Capece A, Romano P. (2008). Molecular identification of yeast species associated with ‘Hamei’—a traditional starter used for rice wine production in Manipur, India. Internatinal Journal of Food Microbiology. 124(2), 115-125.
- Guo C, Zhao C, He P, Lu D, Shen A, Jiang N. (2006). Screening and characterization of yeasts for xylitol production. Journal of Applied Microbiology. 101(5), 1096-1104.
- Altamirano A, Vazquez F, De Figueroa L. (2000). Isolation and identification of xylitol-producing yeasts from agricultural residues. Folia Microbiology. 45(3), 255-258.
- de Mello Lourenço MV, Dini-Andreote F, Aguilar-Vildoso CI, Basso LC. (2014). Biotechnological potential of Candida spp. for the bioconversion of D-xylose to xylitol. African Journal of Microbiology Research. 8(20), 2030-2036.
- Kang HY, Kim YS, Kim GJ, Seo JH, Ryu YW. (2005). Screening and characterization of flocculent yeast, Candida sp. HY200, for the production of xylitol from D-xylose. Journal of Microbial Biotechnology, 15(2), 362-367.